![]() Sticky ends from different BstAPI sites may not be compatible.īclI is typically used at 50-55☌, but is 50% active at 37☌. G C A N N N N N T G C C G T N N N N N A C G Prolonged incubation with NdeI may lead to removal of additional nucleotides.Įfficient cleavage requires at least two copies of the SgrAI recognition sequence. Sticky ends from different Tth111I sites may not be compatible.Īfter cleavage, BamHI-HF® (but not the original BamHI) can remain bound to DNA and alter its electrophoretic mobility. SmaI can be used at 37☌ for brief incubations.Ĭleavage may be enhanced when more than one copy of the XmaI recognition sequence is present.Ĭ T G A A G ( N ) 14 N N G A C T T C ( N ) 14Ĭleavage may be enhanced when more than one copy of the AcuI recognition sequence is present. Sticky ends from different DraIII sites may not be compatible. Sticky ends from different BlpI sites may not be compatible. PaeR7I does not recognize the sequence CTCTCGAG.
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